CO₂ resistant shakers – A selection guide

Mammalian cells in suspension, e.g. for the expression of recombinant proteins or the production of bioreactor starter-cultures (inoculum), are typically cultivated in shake flasks. This process is done in a CO₂-enriched atmosphere with high relative humidity that requires specialized devices. Two systems are commonly used: a) CO₂ resistant shakers that are placed inside a traditional CO₂ incubator and b) CO₂ incubators with an integrated shaker. As there is a significant overlap of application for both systems, the White Paper discusses advantages and disadvantages of both systems and provides tips for the proper selection of a CO₂ resistant shaker.

Aspects discussed in the White Paper: performance for optimized growth conditions, cleanability and contamination prevention, flask capacity, longevity and reliability, device flexibility for various applications, initial investment and running costs.