TSKgel Polymer Based Reversed Phase Columns

Polymer-based reversed phase columns offer the best solution for high pH separations. They are chemically stable from pH 2 to 12, allowing operation at basic pH, where silica- based columns have limited chemical stability. The wider pH range also allows many basic compounds to be analyzed in their uncharged form, thus reducing secondary adsorption and improving peak shape. TSKgel polymer-based columns deliver improved recovery for peptides and proteins due to reduced secondary interactions.

TSKgel Octadecyl-2PW columns are ideally suited for peptides up to 8,000 Da and small proteins due to their 12.5 nm pore size. These columns demonstrate faster analysis than other competitive RP polymeric columns.

TSKgel Octadecyl-4PW columns contain 50 nm pores, allowing for analytical and semi-preparative scale separations of proteins up to 200 kDa.

TSKgel Octadecyl-NPR columns are prepared from nonporous methacrylate particles of uniform 2.5 µm size, which provides high efficiency separations and fast analysis of peptides and proteins at sub-microgram loads.

The 100 nm pore size of the TSKgel Phenyl-5PW RP columns accomodates globular protein samples up to 1.0 × 10⁶ Da.