Protein Purification Handbooks

Cytiva’s Life Sciences business offers principles and methodology handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you’re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab.

Strategies for Protein Purification Handbook

The development of generic purification strategies has revolutionized protein purification by offering speed and simplicity at all scales.
Whether protein purification is performed at microgram or milligram quantities for research or at kilogram to ton quantities for industry, there are proven strategies that can help you apply a systematic approach to purification.

Affinity Chromatography Handbook Collection

Affinity Chromatography Handbooks Vols. 1 to 3 present the most effective and most frequently used strategies for sample preparation and purification of proteins using affinity chromatography in the laboratory. The blend of general guidance and specific examples will be of enormous value to both novice and expert in developing a successful affinity purification strategy for a wide range of biomolecules.

• Affinity Chromatography Vol. 1: Antibodies
• Affinity Chromatography Vol. 2: Tagged Proteins
• Affinity Chromatography Vol. 3: Specific Groups of Biomolecules

 Updated Ion Exchange Chromatography Handbook

The Ion Exchange Chromatography Handbook has been recently updated to include the expanding range of Capto™ chromatography resins for lab- and large-scale protein purification. The update brings Sepharose™ and Capto IEX resins together in the same book and the content will ensure you get the most from your purification runs.
In addition to a new chapter on large-scale purification, the handbook describes each product and how to use them, and provides useful hints and tips for getting great results from your ion exchange chromatography runs. This handbook is an excellent resource on the topic of ion exchange chromatography, whether you are a first-time user or an expert.

Size Exclusion Chromatography Handbook

Since the introduction of Sephadex™ more than 50 yr ago, size exclusion chromatography (SEC) has played a key role in the purification of proteins and enzymes, polysaccharides, nucleic acids, and other biological macromolecules.
Also known as gel filtration, SEC is the simplest and mildest of all chromatography techniques and separates molecules based on differences in size. The handbook will give you all the knowledge and details needed for successful purification runs regardless of whether you use the resin in group separation or high-resolution fractionation mode.

The complete list of available handbooks can be found on https://www.cytivalifesciences.com/en/us/support/handbooks and we welcome you to download the PDF versions to start building your knowledge library. You can also checkout our knowledge videos on YouTube.

https://www.youtube.com/watch?v=B__n4buDycs - The Principle of Affinity Chromatography explained

https://www.youtube.com/watch?v=rPsJo0jHJ7g&t=9s - The Principle of Ion Exchange Chromatography explained

https://www.youtube.com/watch?v=aWtThd13I4I&t=19s - Principles of Gel Filtration (Size Exclusion) Chromatography