Dynal Biotech: Magnetic Bead Chromatography: No Columns Required

07-Jun-2005

Dynal Biotech, a subsidiary of Invitrogen Corporation announced the release of the first two products in its new proteomic preparation range - weak cation exchange (WCX) Dynabeads(r) and reversed phase chromatography (RPC) Dynabeads(r). These novel beads are an excellent alternative to chromatography columns, spin columns and pipette sample preparation technologies. They can fractionate mixed protein samples quickly and easily for downstream analysis such as mass spectrometry.

These superparamagnetic beads can be used to concentrate, prepare and desalt complex protein mixtures. The Dynabeads methodology is not only far quicker and easier to perform, but is also incredibly flexible and can be automated on any device. Dynabeads(r) WCX and Dynabeads(r) RPC Protein are designed for the fractionation of intricate mixtures of protein (e.g. serum, plasma, urine or cell lysates) based on different parameters: Dynabeads(r) WCX carry surface charges which reversibly adsorb oppositely charged proteins and large peptides. Dynabeads RPC Protein have a coating with optimised hydrophobic and hydrophilic components for peptide/protein binding.

The Dynabeads act as a controllable solid phase support, and respond to a magnetic field allowing bound material to be rapidly and precisely separated from salts and other contaminants. Fractions are then released via controlled step-wise elutions. For Dynabeads WCX this is achieved using increasing concentrations of salt or by changing the pH. Dynabeads RPC Protein use hydrophobic interactions and therefore increasing the concentration of acetonitrile will release protein fractions. Since the beads use different properties the two can be used to isolate different fractions from the same sample. This means that they provide a powerful and adaptable preparation protocol for downstream mass spectrum analysis, HPLC and 1D/2D gel electrophoresis.

All Dynal Biotech Dynabeads are highly engineered to provide uniform spheres with even magnetic properties and maximum surface area. They remain dispersed in solution but very quickly move in response to a magnetic field, with no magnetic remanence. The beads have been designed to accommodate many different surface chemistries and have been developed in a number of different sizes to suit different genomic, proteomic and in vitro diagnostic applications.

Other news from the department research and development

These products might interest you

VICI Jour Katalog 15INT

VICI Jour Katalog 15INT by VICI

The VICI Jour Catalog - Accessories for (U)HPLC and Liquid Handling

Capillaries, Tubing, Fittings, Filters, Safety-Products, Tools and much more

chromatography accessories
VICI Valco/Cheminert catalogue 70INT

VICI Valco/Cheminert catalogue 70INT by VICI

Valves and accessories for chromatography, FIA, SFE, SFC - request catalog now

Wow, 284 pages packed with clever solutions and hints for system optimization

chromatography accessories
puriFlash® Ultra Performance Flash & Preparative Purification

puriFlash® Ultra Performance Flash & Preparative Purification by Advion Interchim Scientific

Best-In-Class Flash & Preparative Chromatography for Easy, Efficient & Profitable Purification

Safe and Automated 24/7 Fraction Collection with UV/VIS, ELS and/or MS Detection

flash chromatography systems
Loading...

Most read news

More news from our other portals

All FT-IR spectrometer manufacturers at a glance

See the theme worlds for related content

Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

70+ products
20+ whitepaper
60+ brochures
View topic world
Topic World Chromatography

Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

70+ products
20+ whitepaper
60+ brochures