Bruker Daltonics announces new clinProt Solution by MALDI-TOF and MALDI-TOF/TOF
Bruker Daltonics Inc. today announced that it is entering the tools market for peptide/protein biomarker discovery and clinical proteomics research. Bruker Daltonics' novel ClinProt(TM) solution combines proprietary magnetic bead based sample preparation methods and AnchorChip(TM) technology with high-performance MALDI-TOF and MALDI-TOF/TOF analysis, as well as bioinformatics capabilities for a complete systems solution.
Clinical proteomics research by mass spectrometry is a rapidly growing segment of the overall proteomics market. It is expected to have a profound impact on molecular diagnostics at the peptide and protein level, with the potential to detect, classify and monitor the response to therapy of many diseases, particularly cancer.
Professor Paul Tempst of Memorial Sloan-Kettering Cancer Center (MSKCC) in New York commented: "Our clinical proteomics research here at MSKCC on differentiating various cancer types from controls and from each other via MALDI-TOF analysis of blood serum is advancing very rapidly and is generating a great deal of excitement among many oncologists. MALDI-TOF and MALDI-TOF/TOF analysis provides for the simultaneous measurement of large numbers of polypeptides, which appear to have substantial diagnostic potential for cancer."
THE CLINPROT SYSTEM
Automated sample preparation is a key component of any biomarker discovery system. Bruker Daltonics is introducing a proprietary magnetic bead based system for the selective capture and enrichment of certain classes of peptides and proteins directly from serum, or from other biological samples. This sample preparation technology is automated on Bruker Daltonics' ClinProt liquid handling robots in 96-well microtiter plate formats for throughput and reproducibility. Various magnetic bead types with different chemical or biological functionalities are available for exploratory biomarker studies, including normal phase, anionic, cationic, hydrophobic and metal-ion beads. Magnetic beads with specific biological recognition and capture properties are under development, or can be developed in collaboration with customers.
Dr. Markus Kostrzewa, Director of Bioanalytics at Bruker Daltonics, described the advantages of this approach: "Magnetic bead based enrichment or selective capture in solution is very easy to use. It can be significantly more sensitive and provide higher dynamic range than any surface capture on chips. Moreover, automation is robust, and quantification generally will be improved. Customers can also benefit from the flexibility to implement two-dimensional clean-up procedures on the same robot. Last but not least, as clinical researchers move to larger biomarker studies, measurements using our approach of bead-based extraction from solutions appear to be an order of magnitude less costly than more expensive chip-based methods."
The ClinProt liquid handling robots deposit the samples directly onto our microtiter format AnchorChip targets for analysis by either an AutoFlex(TM) or UltraFlex(TM) MALDI-TOF for the high-resolution, multi-channel detection of large numbers of biomarkers with excellent sensitivity and mass accuracy. Further understanding on significant putative biomarkers can be obtained by de novo sequencing of the selected biomarkers using MALDI-TOF/TOF on the UltraFlex TOF/TOF system.
"Some of our clinical proteomics research customers are now moving beyond biomarker detection and clinical "fingerprinting", and are trying to identify the biomarkers and understand their function scientifically. For this, the additional capabilities of our MALDI-TOF/TOF for high-confidence protein identification from peptide mass fingerprinting or sequence tag analysis, or even de novo sequencing all on the same instrument, is very powerful."
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Topic World Mass Spectrometry
Mass spectrometry enables us to detect and identify molecules and reveal their structure. Whether in chemistry, biochemistry or forensics - mass spectrometry opens up unexpected insights into the composition of our world. Immerse yourself in the fascinating world of mass spectrometry!
Topic World Mass Spectrometry
Mass spectrometry enables us to detect and identify molecules and reveal their structure. Whether in chemistry, biochemistry or forensics - mass spectrometry opens up unexpected insights into the composition of our world. Immerse yourself in the fascinating world of mass spectrometry!
