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Tris
Additional recommended knowledge
Buffering featuresTris has a pKa of 8.3 (at 20 °C), which implies that the buffer is effective pH range between 7.0 and 9.2. Being slightly basic, tris forms an effective buffer for slightly basic solutions, which keeps DNA deprotonated and soluble in water. Tris is commonly combined with EDTA to make "TE buffer" for stabilization and storage of DNA. EDTA binds to divalent cations, particularly magnesium (Mg2+). which is a co-factor for many DNA-modifying enzymes. Buffer detailsBecause of its widespread use of tris buffers, detailed information on its use is available. Some features:
PreparationTris is prepared in two steps from nitromethane via the intermediate (HOCH2)3CNO2 . Reduction of the latter gives tris(hydroxymethyl)aminomethane.[1] Other usesTris is used as an intermediate for the preparation of surface active agents, vulcanization accelerators, and pharmaceuticals, and used as a titrimetric standard. References
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This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Tris". A list of authors is available in Wikipedia. |