TaqI

TaqI is a restriction enzyme isolated from the bacterium Thermus aquaticus in 1978.^[1] It has a recognition sequence of

5'TCGA
3'AGCT

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and makes the cut

5'---T   CGA---3'
3'---AGC   T---5'

References

1. ^ Sato, S. (Feb 1978). "A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus aquaticus, Taq I". J. Biochem (Tokyo) 83 (2): 633–5.