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TCEP



TCEP
IUPAC name tris(2-carboxyethyl)phosphine hydrochloride
Other names TCEP
Identifiers
CAS number 51805-45-9
SMILES OC(CCP(CCC(O)=O)CCC(O)=O)=O
Properties
Molecular formula C9H16O6PCl
Molar mass 286.65 g mol−1
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references

TCEP (tris(2-carboxyethyl)phosphine) is a reducing agent frequently used in biochemistry and molecular biology applications. It is often prepared and used as a hydrochloride salt. TCEP is also available as a stabilized solution at neutral pH and immobilized onto an agarose support to facilitate removal of the reducing agent.

Applications

TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.

Compared to the other two most common agents used for this purpose (dithiothreitol (DTT) and β-mercaptoethanol), TCEP has the advantage of being odourless, more powerful reductant, irreversible reductant, more hydrophilic and more resistant to oxidation in air than is DTT. Finally, it does not reduce metals used for metal chelation purification.

 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "TCEP". A list of authors is available in Wikipedia.
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