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Micrococcal nucleaseMicrococcal Nuclease (S7 Nuclease) is an endo-exonuclease that preferentially digests single-stranded nucleic acids.The rate of cleavage is 30 times greater at the 5' side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved. Additional recommended knowledge
CharacteristicsThe enzyme has a molecular weight of 16.9kDa. The pH optimum is reported as 9.2. The enzyme activity is strictly dependent on Ca2+ and the pH optimum varies according to Ca2+ concentration.[1] The enzyme is therefore easily inacitvated by EDTA. SourceThis enzyme is the extracellular nuclease of Staphylococcus aureus. Two strains, V8 and Foggi, yield almost identical enzymes.[2] A common source is E.coli cells carrying a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal nuclease). Applications
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This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Micrococcal_nuclease". A list of authors is available in Wikipedia. |