MALDI imaging

  MALDI imaging is the use of matrix-assisted laser desorption ionization as an mass spectrometry imaging^[1] technique in which the sample, often a thin tissue section, is moved in two dimensions while the mass spectrum is recorded.^[2]

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Applications

MALDI imaging mass spectrometry involves the visualization of the spatial distribution of proteins, drug candidate compounds and their metabolites, biomarkers or other chemicals within thin slices of sample such as animal tissue or plant.^[3][4][5][6] It is a promising tool for putative biomarker characterisation and drug development. Initially scientists take tissue slices mounted on microscope slides and apply a suitable MALDI matrix to the tissue. Next, the microscope slide is inserted into a MALDI mass spectrometer. The mass spectrometer records the spatial distribution of molecular species such as peptides, proteins or small molecules. Suitable image processing software can be used to import data from the mass spectrometer to allow visualisation and comparison with the optical image of the sample.

See also

• Histology
• Tissue microarray
• Laser capture microdissection

References