Loop-mediated isothermal amplification

Loop mediated isothermal amplification (LAMP) is a single tube technique for the amplification of DNA. It may be combined with a Reverse transcription step to allow the detection of RNA. LAMP is a novel approach to nucleic acid amplification which uses a single temperature incubation therby obviating the need for expensive thermal cyclers. Detection of amplification product can be by photometry for turbidity caused by increasing quantity of Magnesium pyrophosphate in solution or with addition of SYBR green, a colour change can be seen without equipment. Lamp has the potential to be used as a simple screening assay in the field or at the point of care by clinicians.

References

• Notomi T, etal 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Research 28:E63