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EcoRII



EcoRII is an restriction enzyme obtained of Escherichia coli, a Gram-negative bacteria. This enzyme recognizes asymmetric, pseudopalindromic dsDNA sequences, and cuts generating sticky ends.

Cut diagram:

Recognition site Cut results 
5' NNCCWGGNN
3' NNGGWCCNN 

5' NN  CCWGGNN
3' NNGGWCC  NN

W = A or T

References

  • REBASE - The Restriction Enzyme Database
  • Griffiths, J .F. A. et al. (2002): Genética. McGraw-Hill Interamericana, ISBN 84-486-0368-0.
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "EcoRII". A list of authors is available in Wikipedia.
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