EcoRII

EcoRII is an restriction enzyme obtained of Escherichia coli, a Gram-negative bacteria. This enzyme recognizes asymmetric, pseudopalindromic dsDNA sequences, and cuts generating sticky ends.

Additional recommended knowledge

Recognize and detect the effects of electrostatic charges on your balance

Essential Laboratory Skills Guide

Daily Sensitivity Test

Cut diagram:

5' NNCCWGGNN
3' NNGGWCCNN 

5' NN  CCWGGNN
3' NNGGWCC  NN

W = A or T

References

• REBASE - The Restriction Enzyme Database
• Griffiths, J .F. A. et al. (2002): Genética. McGraw-Hill Interamericana, ISBN 84-486-0368-0.