Dioc6

DiOC₆(3) -(3,3′-dihexyloxacarbocyanine iodide) - is a fluorescent dye used for the staining of a cell's ER (endoplasmic reticulum), vesicle membranes and mitochondria. Binding to these structures occurs via the dye's hydrophilic groups. DiOC₆ can be used to label living cells, however they are quickly damaged due to photodynamic toxicity, so cells stained with this dye can only be exposed to light for short periods of time. When exposed to blue light, the dye fluoresces green.

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References

• Koning AJ et al. DiOC6 staining reveals organelle structure and dynamics in living yeast cells. Cell Motil Cytoskeleton. 1993;25(2):111-28.
• Terasaki M. Fluorescent labeling of endoplasmic reticulum. Methods Cell Biol. 1989;29:125-35. Review.