Crista

  Cristae (singular crista) are the internal compartments formed by the inner membrane of a mitochondrion. They are studded with proteins, including ATP synthase and a variety of cytochromes. The maximum surface for chemical reactions to occur is within the mitochondria. This allows cellular respiration (aerobic respiration since the mitochondria requires oxygen) to occur.

Additional recommended knowledge

Safe Weighing Range Ensures Accurate Results

Essential Laboratory Skills Guide

Daily Visual Balance Check

Electron transport chain of the cristae

NADH is split into NAD⁺, H⁺ ions, and electrons by an enzyme. FADH₂ is also split into H⁺ ions, electrons, and FAD. As those electrons travel further through the electron transport chain in the inner membrane, energy is gradually released and used to pump the hydrogen ions from the splitting of NADH and FADH₂ into the space between the inner membrane and the outer membrane (called the intermembrane space), creating an electrochemical gradient. As a result, chemiosmosis occurs, producing ATP from ADP and a phosphate group when ATP synthase harnesses the potential energy from the concentration gradient formed by the amount of H⁺ ions. H⁺ ions passively pass into the mitochondrian matrix by the ATP synthase, and later on help to reform H₂O.

The electron transport chain requires a constant supply of electrons in order to properly function and generate ATP. However, the electrons that have entered the electron transport chain would eventually pile up like cars traveling down a one-way dead-end street. Those electrons are finally accepted by oxygen (O₂), which combine with some of the hydrogen ions from the mitochondrian matrix through ATP synthase and the electrons that had traveled through the electron transport chain. As a result they form two molecules of water (H₂O). By accepting the electrons, oxygen allows the electron transport chain to continue functioning.

The electrons from each NADH molecule can form a total of 2.5 ATPs from ADPs and phosphate groups through the electron transport chain, while each FADH₂ molecule can produce a total of 1.5 ATPs. As a result, the 10 NADH molecules (from glycolysis and the Krebs cycle) and the 2 FADH₂ molecules can form a total of 34 ATPs from this electron transport chain during aerobic respiration. This means that combined with the Krebs Cycle and glycolysis, the efficiency for the electron transport chain is about 65%, as compared to only 3.5% efficiency for glycolysis alone.

Usefulness

The cristae greatly increase the surface area on which the above mentioned reactions take place. If they were absent, the inner membrane would be reduced to a single spherical shape, and with less reaction surface available, the reaction efficiency would be likewise reduced. Therefore, cristae are a necessity for the mitochondria to function efficiently.