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Bottom-up proteomics



Bottom-up proteomics is a method for the identification of proteins in complex mixtures through a combination of two-dimensional gel electrophoresis and mass spectrometry.[1] The name is derived from the analogous bottom-up DNA sequencing.[2] Mass spectrometry analysis consists of a single stage of mass spectrometry for peptide mass fingerprinting or tandem mass spectrometry to obtain a peptide sequence tag.

See also

References

  1. ^ Chait BT (2006). "Chemistry. Mass spectrometry: bottom-up or top-down?". Science 314 (5796): 65–6. doi:10.1126/science.1133987. PMID 17023639.
  2. ^ Watson JD (1990). "The human genome project: past, present, and future". Science 248 (4951): 44–9. PMID 2181665.
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Bottom-up_proteomics". A list of authors is available in Wikipedia.
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