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Alcian blue

Alcian blue

Other names	Alcian blue 8GX, Ingrain blue 1, C.I. 74240
Identifiers
CAS number	33864-99-2
PubChem	3084579
SMILES	CN(C)C(=[N+](C)C)SCC1=CC2=C(C=C1)C3=NC4=NC(=NC5=C6C=C (C=CC6=C([N-]5)N=C7C8=C(C=CC(=C8)CSC(=[N+](C)C)N(C)C)C(=N7) N=C2[N-]3)CSC(=[N+](C)C)N(C)C)C9=C4C=CC(=C9)CSC(=[N+](C)C)N(C)C. [Cl-]. [Cl-].[Cl-].[Cl-].[Cu+2]
InChI	InChI=1/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33- 17-21-37-41(25-33)49-58-45(37)57-46-38-22-18-35(31-75-55 (69(9)10)70(11)12)27-43(38)51(59-46)64-52-44-28-36(32-76- -56(71(13)14)72(15)16)20-24-40(44)48(63-52)62-50-42-26- 34(19-23-39(42)47(60-49)61-50)30-74-54(67(5)6)68(7)8;;;;;/h17- -28H,29-32H2,1-16H3;41H;/q+2;;;;;+2/p-4/fC56H68N16S4.4 Cl.Cu/h;41h;/qm;4*-1;m
Properties
Molecular formula	C₅₆H₆₈Cl_l4CuN₁₆S₄
Molar mass	1298.87 g/mol
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references

Alcian Blue is also a name of a noise pop band which broke up in 2006. [1]

Additional recommended knowledge

Essential Laboratory Skills Guide

Correct Test Weight Handling Guide: 12 Practical Tips

Weighing the right way

Alcian blue (AB), also called Alcian blue 8GX, Ingrain blue 1, and C.I. 74240, is a phthalocyanine dye that contains copper. The dye stains acid mucopolysaccharides and glycosaminoglycans, for which it is one of the most widely used cationic dyes; the stained parts are blue to bluish-green. It can be combined with H&E staining and van Gieson staining methods. It bonds by electrostatic forces with the negatively charged macromolecules. Gradual increases in the electrolyte concentration used to wash the bound dye selectively identifies neutral, sulphated, and phosphated mucopolysaccharides.

Categories: Pigments | Phthalocyanines | Staining dyes